IJAIR NAAS Score : 3.99 ,ISSN : 2319-1473

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IJAIR NAAS Score : 3.99 (2016 - 2020)
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IJAIR NAAS Score : 3.99 (2016 - 2020)
"Submissions Open For Vol. 14,Issue 6, May - June, 2026"


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IJAIR NAAS Score : 3.99 (2016 - 2020)
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Journals Published by TimeLine Publication Pvt. Ltd.

IJECCE , IJEIR , IJAIR,
IJAIM , IJRAS , IJISM ,
IJIRES , IJASM , IJRIES,IJAHVS
https://timelinepublication.com/


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IJAIR NAAS Score : 3.99 (2016 - 2020)
"Submissions Open For Vol. 14,Issue 6, May - June, 2026"


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IJAIR NAAS Score : 3.99 (2016 - 2020)
submit2ijair@yahoo.in , submit@ijair.org


Journals Published by TimeLine Publication Pvt. Ltd.

IJECCE , IJEIR , IJAIR,
IJAIM , IJRAS , IJISM ,
IJIRES , IJASM , IJRIES,IJAHVS
https://timelinepublication.com/


Submissions open

IJAIR NAAS Score : 3.99 (2016 - 2020)
"Submissions Open For Vol. 14,Issue 6, May - June, 2026"


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IJAIR NAAS Score : 3.99 (2016 - 2020)
submit2ijair@yahoo.in , submit@ijair.org


Journals Published by TimeLine Publication Pvt. Ltd.

IJECCE , IJEIR , IJAIR,
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IJIRES , IJASM , IJRIES,IJAHVS
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Influence Factors and Cell Structure Changes Related to Sugarcane Stem Tip Browning in Vitro Culture

Miao Wang; Fen Liao; Liu Yang; Dong-Liang Huang; Li-Tao Yang; Yang-Rui Li
Browning to death is a major limitation for sugarcane tissue culture. This study was mainly carried out to investigate the influence factors which relate to phenolic browning and analyze the relationship between cell structure and browning in sugarcane tissue culture through observing stem tip cellular compartments structure changes in the process of browning. The results showed that different axillary bud obtained obviously different induction survival rate, the first position bud got the highest survival rate, that is to mean the more fresh bud get the higher induction survival rate; the induction survival rate wasn’t effected by different explants collecting season; pre-germination time of 4 weeks resulted higher induction survival rate, and obtained significant difference compared with other pre-germination time treatments. Different buds with different pre-germination time treatments had obvious different total phenolic content, the longer pre-germination time resulted the lower phenolic compounds accumulated in stem tip cells. The nucleus structure deformed, swollen mitochondria stretched, part of the vacuole membrane started to break down at the early browning stage; more serious plasmolysis appeared, a large number of lysosomes appeared in cytoplasm, organelles such as mitochondria completely decomposed, cell membrane, vacuole membrane, nuclear membrane and mitochondrial membrane broke down, cytoplasm completely dissolved, vacuoles mitochondria and other organelles completely decomposed at the later browning stage. This finding inferred that phenolic content, polyphenol oxidase activity as well as nucleus and mitochondria structural distortion and cell membrane broken are factors related to sugarcane stem tip browning in vitro culture. This work provides a reference for improving induction survival rate and lays a solid foundation for further study on mechanism of stem tip browning in vitro culture for sugarcane.
Select Volume / Issue:
Year:
2016
Type of Publication:
Article
Keywords:
Sugarcane; Stem Tip; Browning; Phenolic Content
Journal:
IJAIR
Volume:
4
Number:
4
Pages:
767-772
Month:
January
ISSN:
2319-1473
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