IJAIR NAAS Score : 3.99 ,ISSN : 2319-1473

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IJAIR NAAS Score : 3.99 (2016 - 2020)
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Appraisal of Free Radical Scavenging Activities and Inhibitory Effect on Lipid Peroxidation Related to Phenolic Content of Seed Extracts from Lychee (Litchi chinensis Sonn.)

P. Leelapornpisid; S. Rattanachitthawat; S. Chansakaow
Five cultivars of Lychee (Litchi chinensis Sonn.) seed cultivated in northern Thailand namely: O-Hia, Kwangchoa, Chakrapad, Hong-Huay and Kim Cheng, were collected, dried and ground into powder and then extracted by maceration with 85% ethanol and sequentially partitioned with n-hexane, ethyl acetate and water. Each fraction of the extracts was determined their antioxidant activities in three different assays and total phenolic content. The results revealed that the ethanolic extract (EE) and ethyl acetate (EA) partitioned extract from all the Lychee cultivars exhibited various level of free radical scavenging activities against α, α- diphenyl–ß-picrylhydrazyl (DPPH assay), 2,2'- azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS assay) and inhibitory effect on lipid peroxidation (TBARS assay). Interestingly, we found that all the ethyl acetate (EA) partitioned extract of Lychee seeds possessed potent antioxidant activities, especially that from Kim Cheng presented the highest activity in the three test models which is comparable to vitamin E and gallic acid, with IC50 of 0.09 ± 0.00 mg mL-1 (DPPH), TEAC value of 1.03 ± 0.01 mg Trolox mg sample-1 (ABTS) and IC50 of 0.31 ± 0.04 mg mL-1 (TBARS). This bioactive fraction consists of high total phenolic content (GAE of 0.44 ± 0.00 mg gallic mg sample-1). The chromatographic fingerprint of lychee seed extracts by HPLC at wavelength 280 nm found that the EE and EA extracts presented the same pattern with six major peaks, but different in peak height. The total phenolic content assay and HPLC analysis presented the corresponding data to the antioxidant activities. In the conclusion, Lychee seed extracts can be used as natural source of potent antioxidant that may substitute for synthetic antioxidant in the future. These results will be further investigated for the development into anti-aging cosmetic products.
Select Volume / Issue:
Year:
2015
Type of Publication:
Article
Keywords:
Lychee Seed Extracts; Free Radical Scavenging Activities; Lipid Peroxidation Inhibition; Total Phenolic Content; Chromatographic Fingerprint
Journal:
IJAIR
Volume:
3
Number:
4
Pages:
979-984
Month:
Jan.-Feb.
ISSN:
2319-1473
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